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Anti -PABP Rabbit pAb

Cat. No. GB111164

Brand:Servicebio

Price:230

Specification: 100μl

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Product Information

 

Description

PABP rabbit polyclonal

Protein full name

Polyadenylate-binding protein 1

Synonyms

PABP-1, Poly(A)-binding protein 1, Pabpc1, Pabp1

Immunogen

Recombinant protein corresponding to Mouse PABP

Isotype IgG
Purity Affinity purification
Subcellular location

NucleusCytoplasm

Predicted MW.

71 kDa

Observed MW.

71 kDa

Uniprot ID

P29341, Q9EPH8

 


 

Applications

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Mouse, Rat

Mouse, Rat

Dilution

1: 500-1: 1000

1: 1000-1: 2000

1: 500-1: 1000

Positive tissue

liver, testis, brain

testis

testis

 


 

Background

 

PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
 


 

Images

 

Western blot analysis of PABP (GB111164) at dilution of 1: 1000
Lane 1: HT29 cell lysate
Lane 2: Hela, cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Rat testis tissue lysate
Lane 9: Rat brain tissue lysate

   

Immunohistochemistry analysis of paraffin-embedded mouse testis using PABP (GB111164) at dilution of 1: 1200

   

Immunohistochemistry analysis of paraffin-embedded rat testis using PABP (GB111164) at dilution of 1: 1200

   

Immunofluorescent analysis of paraffin-embedded mouse testis using PABP (GB111164) at dilution of 1: 1200

   

Immunofluorescent analysis of paraffin-embedded rat testis using PABP (GB111164) at dilution of 1: 1200

 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

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