Chinese | English  
Servicebio

TEL

+86 (0) 27 5111 3188

Polyclonals

  1. Home
  2. Products
  3. Primary antibodies
  4. Polyclonals
  5. Content

Anti -MCM2 Rabbit pAb

Cat. No. GB111162

Brand:Servicebio

Price:230

Specification: 100μl

Download Attachment Files
Attachment Download Address: ×
/e/DownSys/DownSoft/?classid=19&id=3539&pathid=0

Product Information

 

Description

MCM2 rabbit polyclonal

Protein full name

DNA replication licensing factor MCM2

Synonyms

Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MCM2

Isotype IgG
Purity Affinity purification
Subcellular location

Nucleus

Predicted MW.

102 kDa

Observed MW.

130 kDa

Uniprot ID

P49736, P97310, D3ZP96

 


 

Applications

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Human, Mouse, Rat

Mouse, Rat

Dilution

1: 500-1: 1000

1: 1000-1: 2000

1: 1000-1: 2000

Positive tissue

thymus

lung cancer, brain, colon cancer, thymus, lung

brain, lung, thymus

 


 

Background

 

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
 


 

Images

 

Western blot analysis of MCM2 (GB111162) at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: SW480 cell lysate
Lane 5: Raji cell lysate
Lane 6: Mouse thymus tissue lysate
Lane 7: Rat thymus tissue lysate

   

Immunohistochemistry analysis of paraffin-embedded human lung cancer using MCM2 (GB111162) at dilution of 1: 2000

   

Immunohistochemistry analysis of paraffin-embedded human colon cancer using MCM2 (GB111162) at dilution of 1: 2000

   

Immunohistochemistry analysis of paraffin-embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000

   

Immunohistochemistry analysis of paraffin-embedded mouse thymus using MCM2 (GB111162) at dilution of 1: 2000

 
   

Immunohistochemistry analysis of paraffin-embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000

 
   

Immunohistochemistry analysis of paraffin-embedded rat lung using MCM2 (GB111162) at dilution of 1: 2000
 
   

Immunofluorescent analysis of paraffin-embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000
 
   

Immunofluorescent analysis of paraffin-embedded mouse lung using MCM2 (GB111162) at dilution of 1: 2000
 
   

Immunofluorescent analysis of paraffin-embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

No relevant information