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Anti -CDKN2A/p16INK4a Rabbit pAb

Cat. No. GB111143

Brand:Servicebio

Price:230

Specification: 100μl

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Product Information

 

Description

CDKN2A rabbit polyclonal

Protein full name
Cyclin-dependent kinase inhibitor 2A

Synonyms

Cyclin-dependent kinase 4 inhibitor A, CDK4I,  p16-INK4a, Cdkn2a, P16ink4a

Immunogen

Recombinant protein corresponding to Human CDKN2A

Isotype IgG
Purity Affinity purification
Subcellular location

Nucleus

Uniprot ID

P51480, Q9R0Z3

 


 

Applications

 

Applications

IHC

IF

Species

Human

Human

Dilution

1: 500-1: 1000

1: 500-1: 1000

Positive tissue

lung cancer, ovarian cancer, breast cancer, colon

breast, colon

 


 

Background

 

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
 


 

Images

 

Immunohistochemistry analysis of paraffin-embedded human lung cancer using CDKN2A (GB111143) at dilution of 1: 1000
 

   

Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using CDKN2A (GB111143) at dilution of 1: 1000
 

   

Immunohistochemistry analysis of paraffin-embedded human breast cancer using CDKN2A (GB111143) at dilution of 1: 1000
 

   

Immunohistochemistry analysis of paraffin-embedded human colon using CDKN2A (GB111143) at dilution of 1: 1000
 

   


Immunofluorescent analysis of paraffin-embedded human breast using CDKN2A (GB111143) at dilution of 1: 1000
 
   


Immunofluorescent analysis of paraffin-embedded human colon using CDKN2A (GB111143) at dilution of 1: 1000
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

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