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Anti -EIF2S2/EIF2B Rabbit pAb

Cat. No. GB111135

Brand:Servicebio

Price:230

Specification: 100μl

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Product Information

 

Description

EIF2S2 rabbit polyclonal

Protein full name

Eukaryotic translation initiation factor 2 subunit 2

Synonyms

eIF-2-beta, Eif2s2, Eukaryotic translation initiation factor 2 subunit beta

Immunogen

Recombinant protein corresponding to Mouse EIF2S2

Isotype IgG
Purity Affinity purification
Subcellular location

Cytoplasm

Predicted MW.

38 kDa

Observed MW.

50 kDa

Uniprot ID

P20042, Q99L45

 


 

Applications

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Human, Rat

Human, Rat

Dilution

1: 500-1: 1000

1: 1000-1: 2000

1: 500-1: 1000

Positive tissue

lung, colon, small intestine

colon, lung, placenta

colon, brain

 


 

Background

 

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.
 


 

Images

 

Western blot analysis of EIF2S2 (GB111135) at dilution of 1: 1000
Lane 1: HT29 cell lysate
Lane 2: L1299 cell lysate
Lane 3: CaCO2 cell lysate
Lane 4: Mouse lung tissue lysate
Lane 5: Mouse colon tissue lysate
Lane 6: Mouse small intestine tissue lysate
Lane 7: Rat lung tissue lysate

   

Immunohistochemistry analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000

   

Immunohistochemistry analysis of paraffin-embedded rat lung using EIF2S2 (GB111135) at dilution of 1: 2000

   

Immunohistochemistry analysis of paraffin-embedded rat placenta using EIF2S2 (GB111135) at dilution of 1: 1000

   

Immunofluorescent analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000
 
   

Immunofluorescent analysis of paraffin-embedded rat brain using EIF2S2 (GB111135) at dilution of 1: 1000
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

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