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Anti -ATG3 Rabbit pAb

Cat. No. GB111131

Brand:Servicebio

Price:230

Specification: 100μl

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Product Information

 

Description

ATG3 rabbit polyclonal

Protein full name

Ubiquitin-like-conjugating enzyme ATG3

Synonyms

Autophagy-related protein 3, Protein PC3-96, APG3-like, hApg3, ATG3, APG3, APG3L

Immunogen

Recombinant protein corresponding to Human ATG3

Isotype IgG
Purity Affinity purification

Predicted MW.

36 kDa

Observed MW.

40 kDa

Uniprot ID

Q6PH08Q8K3M6

 


 

Applications

 

Applications

WB

Species

Human, Mouse

Dilution

1: 500-1: 1000

Positive tissue

THP-2, A20, Hela, Jurkat

 


 

Background

 

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

 


 

Images

 

Western blot analysis of ATG3 (GB111131) at dilution of 1: 1000

Lane 1: THP-2 cell lysate

Lane 2: A20 cell lysate

Lane 3: Hela cell lysate

Lane 4: Jurkat cell lysate

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

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