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Anti -Lck Rabbit pAb

Cat. No. GB111091

Brand:Servicebio

Price:230

Specification: 100μl

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Product Information

 

Description

Lck rabbit polyclonal

Protein full name

Proto-oncogene tyrosine-protein kinase LCK

Synonyms

Leukocyte C-terminal Src kinase,LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Lck, Lsk-t

Immunogen

Recombinant protein corresponding to Mouse Lck

Isotype IgG
Purity Affinity purification
Subcellular location

Cell membrane, Cytoplasm

Predicted MW.

58 kDa

Observed MW.

58 kDa

Uniprot ID

P06239, P06240, Q01621

 


 

Applications

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Mouse, Rat

Mouse, Rat

Dilution

1: 500-1: 1000

1: 1500-1: 3000

1: 1500-1: 3000

Positive tissue

thymus, lymph nodes, spleen

lung, lymph, spleen, thymus

lung, lymph, spleen

 


 

Background

 

LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
 


 

Images

 

Western blot analysis of Lck (GB111091) at dilution of 1: 1000

Lane 1: Jurkat cell lysate
Lane 2: Mouse thymus tissue lysate
Lane 3: Mouse lymph nodes tissue lysate
Lane 4: Mouse spleen tissue lysate
Lane 5: Rat thymus tissue lysate
Lane 6: Rat lymph nodes tissue lysate
Lane 7: Rat spleen tissue lysate
   

Immunohistochemistry analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900
   

Immunohistochemistry analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900
   

Immunohistochemistry analysis of paraffin-embedded rat lymph using Lck (GB111091) at dilution of 1: 1900
   

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 1900
   

Immunohistochemistry analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 1900
   

Immunohistochemistry analysis of paraffin-embedded mouse thymus using Lck (GB111091) at dilution of 1: 1900
   

Immunohistochemistry analysis of paraffin-embedded rat thymus using Lck (GB111091) at dilution of 1: 1900
   

Immunofluorescent analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900
   

Immunofluorescent analysis of paraffin-embedded rat lung using Lck (GB111091) at dilution of 1: 1900
   

Immunofluorescent analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900
   

Immunofluorescent analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 3000
   

Immunofluorescent analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 3000

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.

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