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Anti -SQSTM1/p62 Rabbit pAb

Cat. No. GB11239-1

Brand:Servicebio

Price:230.00

Specification: 100μl

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Product Information

 

Description P62 rabbit polyclonal
Protein full name Sequestosome-1
Synonyms SQSTM1, A170, OSIL, PDB3, ZIP3, p60, 
p62, p62B, FTDALS3, Sequestosome 1, 
NADGP, DMRV
Immunogen Recombinant protein corresponding to 
Mouse P62
Isotype IgG
Purity Affinity purification
Predicted MW. 48 kDa
Observed MW. 62 kDa
Subcellular location Late endosome, Nucleus, PML body,
Lysosome, Endoplasmic reticulum,
Cytosol, Autophagosome, Sarcomere
Uniprot ID Q13501, O08623, Q64337

 


 

Applications

 

Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1500 MCF-7, 293T, liver, muscle
IF Mouse 1:1000-1:2000 NH3T3

 


 

Background
 

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family . Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with SQSTM1, required to recruit ubiquitinated proteins to PML bodies in the nucleus. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD.

 


 

Images

 


Western blot analysis of P62 (GB11239-1) 
at dilution of 1: 1000.
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: A549 cell lysate
Lane 4: Mouse muscle tissue lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Mouse heart tissue lysate
Lane 7: Rat muscle tissue lysate
Lane 8: Rat liver tissue lysate
 

Immunofluorescence analysis of human NH3T3 cells using P62 (GB11239-1) at dilution of 1: 1000
A: Negative control
B:Treated with 50μmol/L Chloroquine overnight 
C:Treated with 1μmol/L rapamycin for 48 hours
D:Treated with 1μmol/L rapamycin for 48 hours and chloroquine overnight

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
 

 

NOTE: This product is intended for research only.