Chinese | English  
Servicebio

TEL

+86 (0) 27 5111 3188

Polyclonals

  1. Home
  2. Products
  3. Primary antibodies
  4. Polyclonals
  5. Content

Anti -PCNA Rabbit pAb

Cat. No. GB11010-1

Brand:Servicebio

Price:230.00

Specification: 100μl

Download Attachment Files
Attachment Download Address: ×
/e/DownSys/DownSoft/?classid=19&id=1317&pathid=0

Product Information

 

 

Description PCNA rabbit polyclonal
Protein full name Proliferating cell nuclear antigen
Synonyms PCNA, ATLD2, proliferating cell nuclear antige
Immunogen Recombinant protein corresponding to Mouse PCNA
Isotype IgG
Purity Affinity purification
Predicted MW. 36 kDa
Observed MW.

36 kDa

Subcellular location Nucleus
Uniprot ID P17918Q15004P04961

 


 

Applications

 

 

Applications IHC, IF WB
Species Human, Mouse, Rat Human
Dilution 1:1000-1:2000 1:500-1:2000
Positive tissue Lung cancer, colon cancer, lung, kidney, testis, heart Hela, HepG2, MCF7, liver cancer

 


 

Background

 

 

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.

 


 

Images

 

 

Western blot analysis of PCNA (GB11010-1) at dilution of 1:1000.

Lane 1: Hela cell lysate

Lane 2: HepG2 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: Human liver tissue lysate

 

   
blob.png

 

Immunohistochemistry analysis of paraffin-embedded human lung cancer using PCNA (GB11010-1) at dilution of 1:2000.

 

   
blob.png

Immunohistochemistry analysis of paraffin-embedded mouse heart using PCNA (GB11010-1) at dilution of 1:2000.

 

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.