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Anti -PCNA Rabbit pAb

Cat. No. GB11010

Brand:Servicebio

Price:230.00

Specification: 100μl

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Product Information

 

 

Description PCNA rabbit polyclonal
Protein full name Proliferating cell nuclear antigen
Synonyms PCNA, ATLD2, proliferating cell nuclear antigen
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse PCNA
Isotype IgG
Purity Affinity purification
Predicted MW. 33 kDa
Observed MW.

33 kDa

Subcellular location Nucleus
Uniprot ID P12004P17918P04961

 


 

Applications

 

 

Applications WB IHC

IF

Species Human, Mouse, Rat

Human, Mouse, Rat

Human, Mouse, Rat
Dilution

1:2000

1:300-1:1000

1:200-1:500

Positive tissue Hela, jurkat, Raw264.7

Liver, lung, spleen

Hela, lung

 


 

Background

 

 

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

      


 

Images

 

 

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Western blot analysis of PCNA (GB11010) at dilution of 1:2000.

Lane 1: hela cell lysate

Lane 2: Jurkat cell lysate

Lane 3: Raw264.7 cell lysate

Lane 4: NRK-52E cell lysate

Lane 5: Human heps tissue lysate

   
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Immunohistochemistry analysis of paraffin-embedded human liver cancer using PCNA (GB11010) at dilution of 1:500.

 

   
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Immunohistochemistry analysis of paraffin-embedded mouse lung using PCNA (GB11010) at dilution of 1:500.

 

   

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Immunohistochemistry analysis of paraffin-embedded rat spleen using PCNA (GB11010) at dilution of 1:500.

   
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Immunofluorescence analysis of paraffin-embedded mice hela tumor using PCNA (GB11010) at dilution of 1:500.

   
blob.png

Immunofluorescence analysis of paraffin-embedded mouse lung using PCNA (GB11010) at dilution of 1:200.

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.