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Anti -Cleaved-Caspase3 Rabbit pAb

Cat. No. GB11009

Brand:Servicebio

Price:230.00

Specification: 100μlThis product is temporarily out of stock.

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Product Information

 

 

Description Cleaved-Caspase3 rabbit polyclonal
Protein full name Caspase-3
Synonyms CASP3, CPP32, CPP32B, SCA-1, caspase 3,  caspase-3, caspase3
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse caspase3
Isotype IgG
Purity Affinity purification
Predicted MW. 17/35 kDa
Observed MW.

17/35 kDa

Subcellular location Cytoplasm
Uniprot ID P70677,P42574,P55213

 


 

Applications

 

 

Applications WB

IHC

IF

Species Human, Mouse, Rat

Mouse

Mouse

Dilution 1:1000 1:200-1:1000

1:200-1:1000

Positive tissue Hela, HepG2, SW480

tumor

tumor

 


 

Background

 

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

 


 

Images

 

 

 

Western blot analysis of Cleaved-Caspase3(GB11009) at dilution of 1:1000.

Lane 1: Hela cell lysate

Lane 2: HepG2 cell lysate

Lane 3: SW480 cell lysate

Lane 4: B16 cell lysate

Lane 5: C6 cell lysate

 

   
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Immunohistochemistry analysis of paraffin-embedded mouse tumor using Cleaved-Caspase3 (GB11009) at dilution of 1:1000

   
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Immunofluorescence analysis of paraffin-embedded mouse tumor using Cleaved-Caspase3 (GB11009) at dilution of 1:500

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.