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Anti-Vimentin Rabbit pAb

Cat. No. GB11192

Brand:Servicebio

Price:230.00

Specification: 100μl

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Product Information

 

 

Description

Vimentin rabbit polyclonal

Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse vimentin 

Isotype IgG
Purity Affinity purification
Predicted MW.

57KDa

Observed MW.

57KDa

Subcellular location

Cytoplasm

Uniprot ID

P08670P20152P31000

 


 

Applications

 

 

Applications

WB

IHC

IF

Species

Human, Mouse, Rat

Human, Mouse, Rat

Human, Mouse, Rat

Dilution

1:500-1:1000

1:500-1:1000

1:200-1:500

Positive tissue

Hela, HepG2, SW480, PC12,lung

Liver cancer, kidney

Kidney, lung

 


 

Background

 

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

 


 

Images

 

 

blob.png

Western blot analysis of Vimentin (GB11192) at dilution of 1:1000.

Lane 1: Hela cell lysate

Lane 2: HepG2 cell lysate

Lane 3: SW480 cell lysate

Lane 4: B16 cell lysate

Lane 5: PC12 cell lysate

Lane 6: Mouse lung tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Vimentin (GB11192) at dilution of 1:1000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Vimentin (GB11192) at dilution of 1:1000

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.