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Anti -beta Tubulin Mouse mAb

Cat. No. GB13017-2

Brand:Servicebio

Price:230.00

Specification: 50μl

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Product Information

 

 

Description

beta Tubulin mouse monoclonal

Protein full name

Tubulin beta chain

Synonyms

TUBB, CDCBM6, M40, OK/SW-cl.56, TUBB1, TUBB5, CSCSC1, tubulin beta class I,Tubulin Beta-5 Chain

Immunogen

KLH conjugated Synthetic peptide corresponding to human β- tubulin

Isotype IgG
Purity Affinity purification

Predicted MW.

55 kDa

Observed MW.

55 kDa
Subcellular location

Cytoplasm

Uniprot ID

P07437P99024P69897

 


 

Applications

 

 

Applications IHC, IF WB  
Species Human, Mouse, Rat Human, Mouse, Rat  
Dilution 1:1000

1:2000-1:5000

 
Positive tissue

Kidney, liver cancer, esophageal cancer

Brain, lung, Hela, HepG2, A549

 

 


 

Background

 

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

            


 

Images

 

 

image.png

Western blot analysis of β- tubulin (GB13017-2) at dilution of 1:2000.

 

Lane 1: Hela cell lysate
 

Lane 2: HepG2 cell lysate
 

Lane 3: A549 cell lysate
 

Lane 4: CNE2 cell lysate
 

Lane 5: Jurkat cell lysate
 

Lane 6: NIH/3T3 cell lysate
 

Lane 7: Raw264.7 cell lysate
 

Lane 8: H9C2 cell lysate

 

   
image.png

Western blot analysis of β- tubulin (GB13017-2) at dilution of 1: 5000.

Lane 1: Hela cell lysate

Lane 2: HepG2 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: SW480 cell lysate

Lane 5: Mouse lung tissue lysate

Lane 6: Mouse brain tissue lysate

Lane 7: Rat lung tissue lysate

Lane 8: Rat brain tissue lysate

   

image.png

Immunohistochemistry analysis of paraffin-embedded rat brain using β- tubulin (GB13017-2) at dilution of 1:1000

   
image.png

Immunofluorescence analysis of paraffin-embedded human esophageal cancer using β- tubulin (GB13017-2) at dilution of 1:1000

 

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.