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Anti -N Cadherin Mouse mAb

Cat. No. GB12135

Brand:Servicebio

Price:230.00

Specification: 100μl

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Product Information

 

 

Description

N Cadherin mouse monoclonal

Protein full name

Cadherin-2

Synonyms

CDH2, CD325, CDHN, CDw325, NCAD, cadherin 2

Immunogen

 

KLH conjugated Synthetic peptide corresponding to Mouse N-cadherin

Isotype IgG

Subcellular location

Membrane

Purity Affinity purification

Predicted MW.

140  kDa

Observed MW

140 kDa

Subcellular location

Cell membrane, Cell junction, Cell surface

Uniprot ID

Q12860, P12960, Q63198

 


 

Applications

 

 

Applications

IHC

IF

WB

Species

Human, Mouse, Rat

Human, Mouse, Rat

Human, Mouse, Rat

Dilution 1: 1000-1: 2000 1: 500-1: 1000

1: 1000-1: 2000

Positive tissue

colon

colon

colon, brain, cerebral cortex 

 


 

Background

 

 

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

   

 


 

Images

 

 

内页.jpg  

Western blot analysis of N Cadherin (GB12135) at dilution of 1: 1000

Lane 1: Hela cell lysate

Lane 2: SH-SY5Y cell lysate

Lane 3: HCT116 cell lysate

Lane 4: Mouse colon tissue lysate

Lane 5: Mouse brain tissue lysate

Lane 6: Mouse cerebral cortex tissue lysate

Lane 7: Rat colon tissue lysate

Lane 8: Rat brain tissue lysate

Lane 9: Rat cerebral cortex tissue lysate

     
image.png  

 

 

Immunohistochemistry analysis of paraffin-embedded human colon usin N Cadherin (GB12135) at dilution of 1: 1500

 

 

     

image.png

 

Immunohistochemistry analysis of paraffin-embedded mouse colon usin N Cadherin (GB12135) at dilution of 1: 1500

     
image.png  

Immunohistochemistry analysis of paraffin-embedded rat colon using N Cadherin (GB12135) at dilution of 1: 1500

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.