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Anti -Brdu Mouse mAb

Cat. No. GB12051

Brand:Servicebio

Price:230.00

Specification: 100μl

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Product Information

 

 

Description

Brdu mouse monoclonal

Protein full name Bromodeoxyuridine

Synonyms

Bromodeoxyuridine;Bromodeoxyuridine;5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation 
Immunogen KLH conjugated BrdU
Isotype IgG
Purity Affinity purification
Subcellular location

Nuclear

Uniprot ID 59-14-3

 


 

Applications

 

 

Applications IHC

IF

 
Species Human, Mouse, Rat

Human, Mouse, Rat

 
Dilution 1:100-1:500

1:100-1:500

 
Positive tissue

Jejunum

Hela

 

 


 

Background

 

 

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. 

     


 

Images

 

 

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Immunofluorescence analysis of Hela cells(treated with 0.03mg/ml BrdU for 40 min )using BrdU (GB12051) at dilution of 1:100

   
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Immunofluorescence analysis of Hela cells(treated with 0.03mg/ml BrdU for 2 h )using BrdU (GB12051) at dilution of 1:100

   
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Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times,the mouse were killed after 24 hours and the jejunum were fixed with 4%PFA)using BrdU (GB12051) at dilution of 1:300

 

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.