RPMI in RPMI-1640 is the abbreviation of Roswell Park Memorial Institute, which stands for Roswell Park Memorial Institute. RPMI-1640 is a cell culture medium developed by the institute. It was originally designed specifically for lymphocyte culture, and has been widely used in the culture of various normal cells and cancer cells, especially the culture of suspension cells, and is one of the most widely used culture media.
This product contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture, without protein or growth factor. 5-10% serum or serum free additives should be added according to the cell type.
This product is sterilized by filtration through a 0.1 μm filter membrane, pH 7.0-7.4, containing 11.1 mM D-glucose, supplemented with 2.05 mM L-glutamine, containing chemical red indicator, containing 25 mM HEPES buffer system, without sodium BT .
HEPES is an excellent biological buffer, which has no toxic effect on cells. The medium added with HEPES can maintain a constant pH range for a long time.