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Cell Culture Medium MEM with NEAA

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Cat No:G4550-500ML
Package: 500ml/Bottle, 25bottles/Carton
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  • G4550-500ML
  • Servicebio

Product content

Name

Cat No.

Size

MEM, with NEAA

G4550-500ML

500 mL

Product description

MEM (Minimum Essential Medium) is one of the most commonly used basal medium for supporting the growth of many suspension and adherent mammalian cells. MEM medium is a more nutrient rich version of basal medium eagle (BME) media, and is suitable for Hela, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts and primary rat astrocytes. There are some other modifications of MEM followed, including with or without non-essential amino acids (including L-Alanine, L-Glutamic acid, L-Asparagine, L-Aspartic acid, L-Proline, L-Serine, and Glycine), addition of ribonucleosides and deoxyribonucleosides, with Earle’s salts for use in a CO2 incubator or with Hank’s salts for use without CO2. This product is made with Earle’s salts, includes non-essential amino acids, no addition of ribonucleosides and deoxyribonucleosides.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this MEM is as follows:

l With 5.5 mM Glucose, non-essential amino acid (NEAA), phenol red

l Without L-glutamine, sodium pyruvate, HEPES.

The complete formulation is available.

Storage

Storage conditions: 2℃ to 8℃, protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture

G4550-500ML MEM Medium 白底-1

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