DMEM/High Glucose,is DMEM (Dulbecco's Modified Eagle Medium) high sugar medium, which is improved on the basis of MEM medium, has been widely used in the culture of various cells presently. DMEM high sugar medium is widely used in cells with fast growth and low adhesion. It can be used for the culture of various mammalian primary cells, such as primary fibroblasts, nerve cells, glial cells, HUVEC, smooth muscle cells, etc. It can also be used for the culture of some cell lines, such as Hela, 293, COS-7 and PC-12, etc.
This product contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture, without protein or growth factor; 5-10% serum or serum free additives should be added according to the cell type.
This product is filtered and sterilized by 0.1μm filter membrane, containing 25 mM glucose, glutamine and sodium BT, without HEPES.
GlutaMAX-I, or L-alanyl-L-glutamine, is a cell culture additive that can replace equimolar L-glutamine in cell culture media. The substance is very stable in aqueous solution and will not degrade spontaneously. Instead, it is slowly degraded by a peptidase secreted by cells to release glutamine, which is then absorbed and utilized by cells.
HEPES is an excellent biological buffer, which has no toxic effect on cells. The medium added with HEPES can maintain a constant pH range for a long time.
|Product description ||DMEM/High Glucose |
|Main Ingredients ||Sodium Bicarbonate/mM ||D-glucose/mM ||L-Glutamine/mM ||Alanyl-Glutamine/mM ||Sodium Pyruvate/mM ||HEPES/mM ||NEAA ||PSP |
|Cat. No. |
|G4510-500ML ||44.04 ||25.0 ||4.0 ||- ||1.0 ||- ||+ ||+ |
|G4511-500ML ||44.04 ||25.0 ||- ||4.0 ||1.0 ||- ||+ ||+ |
|G4512-500ML ||44.04 ||25.0 ||- ||4.0 ||- ||10 ||+ ||+ |
|G4513-500ML ||44.04 ||25.0 ||4.0 ||- ||- ||10 ||+ ||+ |