DMEM/F-12 medium is a 1:1 mixture of DMEM and Ham 'F-12 medium.The improved DMEM/F-12 culture medium is more abundant in nutrients and contains more trace elements. It is widely used in the cultivation of a variety of mammalian cells, and also suitable for the cultivation of mammalian cells with low serum content and the cultivation of clonal density. DMEM/F-12 is also used as a basic medium for the development of serum-free medium
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein or growth factors. It is necessary to add 5-10% serum or serum-free additives according to the cell type.
This product is sterilized by 0.1 μm filter membrane, pH 7.0-7.4, containing 17.5 mM D-glucose, adding 2.5 mM L-glutamine, 0.5 mM sodium BT, containing chemical red indicator, containing 15 mM HEPES buffer system.
HEPES is a commonly used biological buffer, which can keep the culture medium in a constant pH range for a long time and effectively prevent large fluctuations in the pH of the culture fluid from adversely affecting cell growth.