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Anti -UBE2M/UBC12 Rabbit pAb primary antibody Western blot

Specification: 100uL
  • GB112655
  • Servicebio
  • Servicebio

Description

Product Information


Description

UBE2M/UBC12 rabbit polyclonal

Protein full name

NEDD8-conjugating enzyme Ubc12

Synonyms

NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M, Ube2m, Ubc-rs2, Ubc12, hUbc12, yeast UBC12 homolog

Immunogen

Recombinant protein corresponding to

Mouse UBE2M/UBC12

Isotype

IgG

Purity

Affinity purification

Predicted MW.

21 kDa

Observed MW.

21 kDa

Uniprot ID

P61081, P61082




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

testis, brain




Background


Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.




Images


图片 1.png

Western blot analysis of UBC12 (GB112655) at dilution of 1: 2000

Lane 1: 293 cell lysate

Lane 2: Jurkat cell lysate

Lane 3: HepG2 cell lysate

Lane 4: MCF7 cell lysate

Lane 5: NIH3T3 cell lysate

Lane 6: Mouse testis tissue lysate

Lane 7: Mouse brain tissue lysate

Lane 8: Rat testis tissue lysate

Lane 9: Rat brain tissue lysate




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE: This product is intended for research only.

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