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Anti -PCNA Mouse MAb for Immunohistochemistry Western Blot Research Antibody

Specification: 100uL
  • GB12010
  • Servicebio
  • Servicebio

Description

Product Information


Description

PCNA mouse monoclonal

Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse PCNA 

Isotype

IgG

Purity

Nucleus

Subcellular location

29 kDa

Predicted MW.

36 kDa

Observed MW.

Affinity purification

Uniprot ID

P12004P17918P04961




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

testis, placenta

IHC

Mouse, Rat

1: 2000-1: 4000

testis, colon




Background


Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.




Images



Western blot analysis of PCNA (GB12010) 

at dilution of 1: 1000

Lane 1: HeLa cell lysate

Lane 2: HepG2 cell lysate

Lane 3: A549 cell lysate

Lane 4: NIH3T3 cell lysate

Lane 5: C2C12 cell lysate

Lane 6: Mouse testis tissue lysate

Lane 7: Mouse placenta tissue lysate

Lane 8: Rat testis tissue lysate

Lane 9: Rat placenta tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse testis using 

PCNA (GB12010) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat testis using 

PCNA (GB12010) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse colon using 

PCNA (GB12010) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE: This product is intended for research only.

downLoadImgSlice 10Laboratory(1)Slice 13Slice 12

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