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GB112408 Anti -Cardiac Troponin I Tnnc1 CMH7 Cardiac Troponin I Rabbit Polyclonal Antibody

Immunogen: KLH conjugated Synthetic peptidecorresponding to Human Cardiac Troponin I
Isotype: IgG
Purity: Affinity purification
ul:
  • GB112408
  • Servicebio

Description

Product Information

 

Description

Cardiac Troponin I rabbit polyclonal

Protein full name

Cardiac Troponin I

Synonyms

Cardiac troponin I, TNNI3, TNNC1,cTnI, CMH7, cardiac muscle, TNNI3,Troponin I type 3 cardiac , cardiac type,CTnI 

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cardiac Troponin I

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton

Predicted MW.

24 kDa

Observed MW.

28 kDa

Uniprot ID

P19429, P48787, P23693

 


 

Applications

 

Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 250-1: 500

heart

IHC

Mouse, Rat

1: 100-1: 200

heart

IF

Mouse, Rat

1: 100-1: 200

heart

 


 

Background

 

Troponin I is a cardiac and skeletal muscle protein useful in the laboratory diagnosis of heart attack. Troponin I is a part of the troponin protein complex, where it binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.

 


 

Images

 

Western blot analysis of cTnI 

(GB112408) at dilution of 1: 500

Lane 1: Mouse heart tissue lysate

Lane 2: Mouse skeletal muscle tissue lysate

Lane 3: Rat heart tissue lysate

Lane 4: Rat skeletal muscle tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse heart using 

cTnI (GB112408) at dilution of 1: 200

Immunohistochemistry analysis of paraffin-embedded rat heart using 

cTnI (GB112408) at dilution of 1: 200


A

B

C

Immunofluorescent analysis of paraformaldehyde-fixed mouse heart using 

cTnI (GB112408) at dilution of 1: 200

A: DAPI nuclear stain (blue)

B: Mouse heart lysate labeling cTNl with GB112408, followed by Goat anti-rabbit IgG (red)

C: Merged

A

B

C

Immunofluorescent analysis of paraformaldehyde-fixed Rat heart using 

cTnI (GB112408) at dilution of 1: 200

A: DAPI nuclear stain (blue)

B: Rat heart lysate labeling cTNl with GB112408, followed by Goat anti-rabbit IgG (red)

C: Merged


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

 

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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