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GB112098 Anti -Cyclin B1 Cycb1 CCNB Rabbit Polyclonal Antibody for WB IHC IF

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1
Isotype: IgG
Purity: Affinity purification
 
ul:
  • GB112098
  • Servicebio

Description

Product Information


Description

Cyclin B1 rabbit polyclonal

Protein full name

G2/mitotic-specific cyclin-B1

Synonyms

Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1

Isotype

IgG

Purity

Affinity purification

Subcellular location

CytoplasmCytoskeletonNucleus

Predicted MW.

48 kDa

Observed MW.

55 kDa

Uniprot ID

P14635, P24860, P30277



Applications



Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

testis, colon, spleen

IHC

Human, Mouse, Rat

1: 1500-1: 3000

colon cancer, skin, colon

IF

Human, Mouse, Rat

1: 400-1: 800

lung cancer, cervical cancer, testis, colon



Background


The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.




Images




Western blot analysis of Cyclin B1 (GB112098) at dilution of 1: 1000

Lane 1: Jurkat cell lysate

Lane 2: 293T cell lysate

Lane 3: Mouse testis tissue lysate

Lane 4: Mouse colon tissue lysate

Lane 5: Rat testis tissue lysate

Lane 6: Rat colon tissue lysate

Lane 7: Rat spleen tissue lysate

Immunohistochemistry analysis of paraffin-embedded human colon cancer using Cyclin B1 (GB112098) at dilution of 1: 3000

Immunohistochemistry analysis of paraffin-embedded human skin using

Cyclin B1 (GB112098) at dilution of 1: 3000

Immunohistochemistry analysis of paraffin-embedded mouse colon using

Cyclin B1 (GB112098) at dilution of 1: 3000

Immunohistochemistry analysis of paraffin-embedded rat colon using

Cyclin B1 (GB112098) at dilution of 1: 3000

Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using Cyclin B1 (GB112098) at dilution of 1: 800

Immunofluorescent analysis of paraformaldehyde-fixed human cervical cancer using Cyclin B1 (GB112098) at dilution of 1: 800

Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using 

Cyclin B1 (GB112098) at dilution of 1: 800

Immunofluorescent analysis of paraformaldehyde-fixed rat testis using 

Cyclin B1 (GB112098) at dilution of 1: 800


Immunofluorescent analysis of paraformaldehyde-fixed rat colon using 

Cyclin B1 (GB112098) at dilution of 1: 800





Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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