Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.
Western blot analysis of Twinkle (GB112301) at dilution of 1: 1000
Lane 1: Rat pancreas tissue lysate
Immunohistochemistry analysis of paraffin-embedded mouse kidney using
Twinkle (GB112301) at dilution of 1: 3000
Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using
Twinkle (GB112301) at dilution of 1: 800
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.