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Product Information
Description | Twinkle rabbit polyclonal |
Protein full name | Twinkle protein, mitochondrial |
Synonyms | Progressive external ophthalmoplegia 1 protein homolog, T7 gp4-like protein with intramitochondrial nucleoid localization, T7-like mitochondrial DNA helicase, Twinkle mtDNA helicase, Twnk, Peo1 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Twinkle |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Mitochondrion |
Predicted MW. | 77 kDa |
Observed MW. | 72 kDa |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Rat | 1: 500-1: 1000 | pancreas |
IHC | Mouse, Rat | 1: 500-1: 1000 | kidney |
IF | Mouse, Rat | 1: 800-1: 1600 | kidney |
Background
Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.
Images
| Western blot analysis of Twinkle (GB112301) at dilution of 1: 1000 Lane 1: Rat pancreas tissue lysate |
 | Immunohistochemistry analysis of paraffin-embedded mouse kidney using Twinkle (GB112301) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using Twinkle (GB112301) at dilution of 1: 800 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
