English
Product Information
| Description | beta Actin rabbit polyclonal |
Protein full name | Actin, cytoplasmic 1 |
Synonym | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
| Isotype | IgG |
| Predicted MW. | 42 kDa |
Observed MW. | 45 kDa |
Purity | Affinity purification |
Uniprot ID | P60710 |
Applications
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | lung, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | colon cancer, liver |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549, etc. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility .
Images
| Western blot analysis of beta-Actin (GB11001) at dilution of 1: 1000 |
| Immunofluorescence of paraffin embedded human colon cancer using beta-Actin (GB11001) at dilution of 1: 1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:This product is intended for research only.
