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Anti -GAPDH Rabbit PAb Western Blot Loading Control Primary Antibody

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specification:100ul
applications: WB IHC IF
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  • GB11002
  • Servicebio
  • Servicebio

,Product Information


Description

GAPDH rabbit polyclonal

Protein full name

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Isotype IgG
Purity

Affinity purification

Subcellular location
Cytoplasm,Nucleus,Membrane
Predicted MW.

36 kDa

Observed MW.

36 kDa

Uniprot ID

P16858




Applications

Species

dilution

Positive tissue


WB

Human, Mouse, Rat

1:1000-1:2000

brain, spleen, kidney, liver

IHC/IF
Human, Mouse, Rat 1:500-1:1000 kidney, testis


Background


Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.




Images




图片 2.png

Western blot analysis of GAPDH (GB11002) at dilution of 1: 1000

图片 3.png

Western blot analysis of GAPDH (GB11002) at dilution of 1: 1000


1.20220309_GAPDH_GB11001 M kindey d500_20x_IHC(1)

Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB11002) at dilution of 1: 500

2.20220309_GAPDH_GB11001 R testis d500_20x_IHC

Immunohistochemistry of paraffin embedded rat testis using GAPDH (GB11002) at dilution of 1: 500




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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Cell death & disease

IF 8.469    Time:2021   Cell death & disease

Hong, N. et aThe transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.Cell death & disease 12,393-393(2021).PMID 33846290 Application: IF  IF 8.469

Journal of inflammation research

IF 6.922    Time:2021   Cell death & disease

Chen, H. et alCapsaicin Protects Against Lipopolysaccharide-Induced Acute Lung Injury Through the HMGB1/NF-κB and PI3K/AKT/mTOR Pathways. Journal of inflammation research 14,5291-5304(2021).PMID 34703269 Application: WB IF 6.922

Frontiers in cell and developmental biology

IF 6.684    Time:2021   Frontiers in cell and developmental biology

Jiang, D. et alProlyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet.Frontiers in cell and developmental biology 9,628143-628143(2021).PMID 34095107 Application: WB IF 6.684

Frontiers in oncology

IF 6.244    Time:2021   Frontiers in oncology

Wang, C. et alER-α36 Promotes the Malignant Progression of Cervical Cancer Mediated by Estrogen via HMGA2.Frontiers in oncology 11,712849-712849(2021).PMID 34336701 Application: WB IF 6.244

Frontiers in pharmacology

IF 5.81    Time:2021   Frontiers in pharmacology

Ji, Y. et alXiaoyao Pills Ameliorate Depression-like Behaviors and Oxidative Stress Induced by Olfactory Bulbectomy in Rats via the Activation of the PIK3CA-AKT1-NFE2L2/BDNF Signaling Pathway.Frontiers in pharmacology 12,643456-643456(2021).PMID 33935736 Application: WB IF 5.81

Frontiers in pharmacology

IF 5.81    Time:2021   Frontiers in pharmacology

Chang, Z.-P. et alShaoyao-Gancao Decoction Ameliorates the Inflammation State in Polycystic Ovary Syndrome Rats via Remodeling Gut Microbiota and Suppressing the TLR4/NF-κB Pathway.Frontiers in pharmacology 12,670054-670054(2021).PMID 34054541 Application: WB IF 5.81

Biomolecules

IF 4.879    Time:2021   Biomolecules 

Du, X.-L. et alGlucocorticoid Receptor Alpha Targets SLC2A4 to Regulate Protein Synthesis and Breakdown in Porcine Skeletal Muscle Cells.Biomolecules 11,721(2021).PMID 34065777 Application: WB IF 4.879

OncoTargets and therapy

IF 4.147    Time:2021   OncoTargets and therapy 

Huang, W., Zhu, J., Shi, H., Wu, Q. & Zhang, CITGA2 Overexpression Promotes Esophageal Squamous Cell Carcinoma Aggression via FAK/AKT Signaling Pathway.OncoTargets and therapy 14,3583-3596(2021).PMID 34113124 Application: WB IF 4.147

Annals of translational medicine

IF 3.932    Time:2021   Annals of translational medicine

Lu, Y. et alIdentification of the distinctive role of DPT in dilated cardiomyopathy: a study based on bulk and single-cell transcriptomic analysis.Annals of translational medicine 9,1401-1401(2021).PMID 34733953 Application: WB IF 3.932

Molecular medicine reports

IF 2.952    Time:2022   Molecular medicine reports

Wu, Q., Li, Q., Zhu, W., Zhang, X. & Li, HEpsin 3 potentiates the NF‑κB signaling pathway to regulate apoptosis in breast cancer.Molecular medicine reports 25,15(2022).PMID 34779498 Application: WB IF 2.952



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