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Anti -PCNA Rabbit pAb for Immunoblotting western blot

Specification:100ul
  • GB11010
  • Servicebio
  • Servicebio

Description


Product Information


Description

PCNA rabbit polyclonal

Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse PCNA

Isotype

IgG

Predicted MW.

29 kDa

Observed MW.

36 kDa

Purity

Affinity purification

Subcellular location

Nucleus

Uniprot ID

P12004, P17918, P04961




Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 2000

HeLa, jurkat, Raw264.7

IHC

Human, Mouse, Rat

1: 300-1: 1000

liver, lung, spleen

IF

Human, Mouse, Rat

1: 200-1: 500

Hela, lung




Background


Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.




Images



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Western blot analysis of PCNA (GB11010) at dilution of 1: 2000.

Lane1: HeLa cell lysate

Lane2: Jurkat cell lysate

Lane3: Raw264.7 cell lysate

Lane4: NRK-52E cell lysate

Lane5: Human heps tissue lysate


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Immunohistochemistry analysis of paraffin-embedded human liver cancer using PCNA (GB11010) at dilution of 1: 500.

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Immunohistochemistry analysis of paraffin-embedded mouse lung using PCNA (GB11010) at dilution of 1: 500.


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Immunohistochemistry analysis of paraffin-embedded rat spleen using PCNA (GB11010) at dilution of 1:500.

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Immunofluorescence analysis of paraffin-embedded mice hela tumor using PCNA (GB11010) at dilution of 1:500.

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Immunofluorescence analysis of paraffin-embedded mouse lung using PCNA (GB11010) at dilution of 1:200.




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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