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GB112131 WB IHC IF Anti -Slow Skeletal Myosin Heavy Chain Rabbit Polyclonal Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to MYH7
Isotype: IgG
Purity: Affinity purification
ul:
  • GB112131
  • Servicebio

Description

Product Information


Description

Slow Skeletal Myosin Heavy chain rabbit polyclonal

Protein full name

Myosin-7

Synonyms

Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, MyHC-beta, MYH7, MYHCB, CMD1S, SPMM, SPMD, MPD1, Beta myosin heavy chain

Immunogen

KLH conjugated Synthetic peptide

corresponding to MYH7

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

223 kDa

Observed MW.

223 kDa

Uniprot ID

P12883, Q91Z83, P02564





Applications


Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 300-1: 600

myocardium

IHC

Human, Mouse, Rat

1: 500-1: 1000

skeletal muscle

IF

Human, Mouse, Rat

1: 500-1: 1000

skeletal muscle




Background


This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading.




Images


Western blot analysis of MYH7 (GB112131)

at dilution of 1: 600

Lane 1: Mouse myocardium tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using MYH7 (GB112131) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat skeletal muscle using

MYH7 (GB112131) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat skeletal muscle using

MYH7 (GB112131) at dilution of 1: 1000

Immunofluorescent analysis of paraformaldehyde-fixed mouse skeletal muscle using MYH1 (GB112130, red light) and MYH7 (GB112131, green light) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using MYH1 (GB112130, red light) and MYH7 (GB112131, green light) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using MYH1 (GB112130, red light) and MYH7 (GB112131, green light) at dilution of 1: 1000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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