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Anti -PRPF4 Rabbit PAb for IHC IF Western Blot Primary Antibody

Specification: 100uL

Applications

: WB IHC IF
  • GB112280
  • Servicebio
  • Servicebio

Description

Product Information

 

Description

PRPF4 rabbit polyclonal

Protein full name

U4/U6 small nuclear ribonucleoprotein Prp4

Synonyms

U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4, Prpf4, Prp4p, HPRP4P

Immunogen

Recombinant protein corresponding to Mouse PRPF4

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

58 kDa

Observed MW.

58 kDa

Uniprot ID

O43172, Q9DAW6


 

Applications

 

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, spleen

IHC

Human, Mouse, Rat

1: 1000-1: 2000

ovarian cancer, breast cancer, substantia nigra, lymph node, bladder, nephritis, lung, liver, striatum, heart

IF

Human, Mouse, Rat

1: 500-1: 1000

lung, lymph node, ovary, brain, bladder, liver, kidney, heart


 

Background

 

PRPF4, also named as U4/U6 small nuclear ribonucleoprotein Prp4, is a 522 amino acid protein, which contains seven WD repeats. PRPF4 localizes in the Nucleus speckle. PRPF4 participates in pre-mRNA splicing and is a part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.PRPF4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes.

 


 

Images

 

Western blot analysis of PRPF4 (GB112280) 

at dilution of 1: 1000

Lane 1: HeLa cell lysate

Lane 2: C6 cell lysate

Lane 3: RAW264.7 cell lysate

Lane 4: MCF7 cell lysate

Lane 5: A431 cell lysate

Lane 6: Mouse brain tissue lysate

Lane 7: Rat spleen tissue lysate

Lane 8: Rat brain tissue lysate

Lane 9: Rat spleen tissue lysate

Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using 112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded human breast cancer using 112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse substantia nigra using 112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse lymph node using 112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse bladder using

112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse nephritis using

112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat lung using

112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat liver using

112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat striatum using

112280 (GB112280) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat heart using

112280 (GB112280) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using 

PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed mouse lymph node using PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed mouse ovary using 

PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using 

PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed mouse bladder using PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed rat liver using 

PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using 

PRPF4 (GB112280) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed rat heart using 

PRPF4 (GB112280) at dilution of 1: 500

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

 

NOTE: This product is intended for research only.

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