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Anti -Cytokeratin 8 Rabbit pAb for WB IHC IF primary antibody

Specification: 100uL
Applications:WB、 IHC 、IF
  • GB11231
  • Servicebio
  • Servicebio

Description

Product Information


Description

Cytokeratin 8 rabbit polyclonal

Protein full name

Keratin, type II cytoskeletal 8

Synonyms

KRT8, CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, keratin 8

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse Cytokeratin 8

Isotype

IgG

Purity

Affinity purification

Predicted MW.

54 kDa

Observed MW.

54 kDa

Subcellular location

Cytoplasm, Nucleus

Uniprot ID

P05787, P11679, Q10758




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000

HepG2, A549, MCF7, colon, liver

IHC

Human, Mouse, Rat

1: 200-1: 1000

colon cancer,

stomach cancer,

lung stomach

IF

Human, Mouse, Rat

1: 200-1: 1000

colon cancer,

stomach cancer,

lung stomach




Background


This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.



Images



图片 4.png

Western blot analysis of Cytokeratin 8 (GB11231)at dilution of 1: 1000.

Lane 1: HepG2 cell lysate

Lane 2: A549 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: Mouse liver tissue lysate

Lane 5: Mouse kidney tissue lysate

Lane 6: Mouse colon tissue lysate

Lane 7: Rat liver tissue lysate

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Immunohistochemistry analysis of paraffin-embedded human colon using Cytokeratin 8 (GB11231) at dilution of 1: 200.

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Immunohistochemistry analysis of paraffin-embedded human colon cancer using Cytokeratin 8 (GB11231) at dilution of 1:200.

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Immunohistochemistry analysis of paraffin-embedded human stomach cancer using Cytokeratin 8 (GB11231) at dilution of 1:200.

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Immunohistochemistry analysis of paraffin-embedded mouse lungusing Cytokeratin 8 (GB11231) at dilution of 1:200.

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Immunohistochemistry analysis of paraffin-embedded rat stomach using Cytokeratin 8 (GB11231) at dilution of 1:800.




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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