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Anti -Cytokeratin 18 Rabbit pAb for IHC IF western blot primary antibody

Specification: 100uL
applications:WB、IHC 、IF
  • GB11232
  • Servicebio
  • Servicebio

Description

Product Information


Description

Cytokeratin 18 rabbit polyclonal

Protein full name

Keratin, type I cytoskeletal 18

Synonyms

KRT18,CK-18,CK18,CYK18,K18,keratin 18

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse Cytokeratin 18

Isotype

IgG

Predicted MW.

48 kDa

Observed MW.

48 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm,Nucleus

Uniprot ID

P05783, P05784, Q5BJY9




Applications


applications

species

dilution

Positive tissue

WB

Human,Mouse,Rat

1:1000

Colon,A548,HepG2

IHC

Human,Mouse,Rat

1:300-1:1000

Kidney,stomach

IF

Human,Mouse,Rat

1:200-1:1000

Breast cancer




Background


KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.



Images



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Western blot analysis of Cytokeratin 18 (GB11232) at dilution of 1:1000.

Lane 1:A549 cell lysate

Lane 2:HepG2 cell lysate

Lane 3:MCF7 cell lysate

Lane 4:SW480 cell lysate

Lane 5:Mouse colon tissue lysate

Lane 6:Mouse colon tissue lysate

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 18 (GB11232) at dilution of 1:1000

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Immunohistochemistry analysis of paraffin-embedded human liver using Cytokeratin 18 (GB11232) at dilution of 1:1000

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Immunohistochemistry analysis of paraffin-embedded human stomach using Cytokeratin 18 (GB11232) at dilution of 1:1000

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Immunohistochemistry analysis of paraffin-embedded human kidney using Cytokeratin 18 (GB11232) at dilution of 1:1000

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Immunohistochemistry analysis of paraffin-embedded mouse liver using Cytokeratin 18 (GB11232) at dilution of 1:1000

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using Cytokeratin 18 (GB11232) at dilution of 1:1000

blob.png

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Cytokeratin 18 (GB11232) at dilution of 1:1000

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Immunohistochemistry analysis of paraffin-embedded mouse uterus using Cytokeratin 18 (GB11232) at dilution of 1:1000


Immunohistochemistry analysis of paraffin-embedded rat lung using Cytokeratin 18 (GB11232) at dilution of 1:1000

Immunohistochemistry analysis of paraffin-embedded rat liver using Cytokeratin 18 (GB11232) at dilution of 1:1000

Immunohistochemistry analysis of paraffin-embedded rat kidney using Cytokeratin 18 (GB11232) at dilution of 1:1000

Immunohistochemistry analysis of paraffin-embedded rat pancreas using Cytokeratin 18 (GB11232) at dilution of 1:1000

Immunofluorescence analysis of paraffin-embedded human breast cancer using Cytokeratin 18 (GB11232) at dilution of 1:500




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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