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GB11239-1 Anti -SQSTM1 p62B NADGP Sequestosome 1 FTDALS3 Rabbit Polyclonal Antibody

Immunogen: Recombinant protein corresponding to Mouse P62
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11239-1
  • Servicebio

Description

Product Information


Description

P62 rabbit polyclonal

Protein full name

Sequestosome-1

Synonyms

SQSTM1, A170, OSIL, PDB3, ZIP3, p60,

p62, p62B, FTDALS3, Sequestosome 1,

NADGP, DMRV

Immunogen

Recombinant protein corresponding to

Mouse P62

Isotype

IgG

Purity

Affinity purification

Predicted MW.

48 kDa

Observed MW.

62 kDa

Subcellular location

Late endosome, Nucleus, PML body,

Lysosome, Endoplasmic reticulum,

Cytosol, Autophagosome, Sarcomere

Uniprot ID

Q13501, O08623, Q64337




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1500

liver, muscle

IF

Mouse

1: 1000-1: 2000

NIH3T3




Background

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family . Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with SQSTM1, required to recruit ubiquitinated proteins to PML bodies in the nucleus. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD.




Images


GB11239-1.png

Western blot analysis of P62 (GB11239-1) at dilution of 1: 1000.

Lane 1: MCF-7 cell lysate

Lane 2: 293T cell lysate

Lane 3: A549 cell lysate

Lane 4: Mouse muscle tissue lysate

Lane 5: Mouse liver tissue lysate

Lane 6: Mouse heart tissue lysate

Lane 7: Rat muscle tissue lysate

Lane 8: Rat liver tissue lysate

20200825092626.jpg

Immunofluorescent analysis of 4% paraformaldehyde-fixed NIH3T3 cell using P62 (GB11239-1) at dilution of 1: 1000

A: negative control

B: treated with 50 μ mol/L Chloroquine overnight

C: treated with 1 μ mol/L Rapamycin for 48 hours

D: treated with 1 μ mol/L Rapamycin overnight and Chloroquine overnight




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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