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GB11243 Anti -Hsp60 HuCHA60 CPN60 60 KDa Chaperonin Rabbit Polyclonal Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse HSP60
Isotype: IgG
Purity: 60 kDa
ul:
  • GB11243
  • Servicebio

Description

Product Information


Description HSP60 rabbit polyclonal
Protein full name 60 kDa heat shock protein, mitochondrial
Synonyms HSP60, HSP-60, HuCHA60, CPN60,
60 KDa Chaperonin, P60 Lymphocyte Protein, Heat Shock Protein 60
Immunogen KLH conjugated Synthetic peptide
corresponding to Mouse HSP60
Isotype IgG
Purity 60 kDa
Predicted MW. 60 kDa
Observed MW. Affinity purification
Subcellular location Mitochondrion matrix
Uniprot ID P10809, P63039, P63038




Applications


Applications Species Dilution Positive Tissue
WB Human,
Mouse, Rat
1:3000-1:10000 spleen, brain, U87,
U251, RGC-5, SH-SY5Y
IHC, IF Human,
Mouse, Rat
1: 500-1: 1000 colon, kidney
IEM Mouse 1: 50 liver, heart, pancreas




Background


Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. Through the extensive study of groEL, HSP60’s bacterial homolog, HSP60 has been deemed essential in the synthesis and transportation of essential mitochondrial proteins from the cell's cytoplasm into the mitochondrial matrix. Further studies have linked HSP60 to diabetes, stress response, cancer and certain types of immunological disorders.




Images



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Western blot analysis of HSP60 (GB11243) at dilution of 1: 5000.
Lane 1: U87 cell lysate
Lane 2: U251 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: RGC-5 cell lysate
Lane 5: Raw264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Rat spleen tissue lysate
Lane 9: Rat brain tissue lysate


Immunohistochemistry analysis of paraffin-embedded human colon using HSP60 (GB11243) at dilution of 1: 1000

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Immunohistochemistry analysis of paraffin-embedded rat kidney using HSP60 (GB11243) at dilution of 1: 1000



Immunohistochemistry analysis of paraffin-embedded mouse kidney using HSP60 (GB11243) at dilution of 1: 1000

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Immunoelectron microscopy analysis of LR white resin-embedded mouse liver using HSP60 (GB11243) at dilution of 1: 50.
A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.



Immunoelectron microscopy analysis of LR white resin-embedded mouse liver using HSP60 (GB11243) at dilution of 1: 50.
A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.

Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using HSP60 (GB11243) at dilution of 1: 50.
A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.

Immunoelectron microscopy analysis of LR white resin-embedded mouse pancreas using HSP60 (GB11243) at dilution of 1: 50.
A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.

Immunoelectron microscopy analysis of LR white resin-embedded mouse pancreas using HSP60 (GB11243) at dilution of 1: 50.
A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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