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Anti -Nanog Rabbit pAb for WB IHC IF

Specification: 100uL
  • GB11331
  • Servicebio
  • Servicebio

Description

Product Information


Description

Nanog rabbit polyclonal

Protein full name

Homeobox protein NANOG

Synonyms

NANOG, Nanog, Nanog homeobox, hNanog, Homeobox protein NANOG

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Nanog

Isotype

IgG

Predicted MW.

42 kDa

Observed MW.

42 kDa

Purity

Affinity purification

Subcellular location

Nucleus

Uniprot ID

Q80Z64




Applications


applications

species

dilution

Positive tissue

WB

Mouse, Rat

1: 500-1: 1000

fetal brain, ovary

IHC

Mouse, Rat

1: 500-1: 1000

testis

IF

Mouse, Rat

1: 500-1: 1000

testis




Background


Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.




Images



图片 1.png

Western blot analysis of Nanog (GB11331) at dilution of 1:1000.

Lane 1: Fetal mouse brain tissue lysate

Lane 2: Mouse ovary tissue lysate

Lane 3: Fetal rat brain tissue lysate

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Immunohistochemistry analysis of paraffin-embedded rat testis using Nanog (GB11331) at dilution of 1:500

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Immunohistochemistry analysis of paraffin-embedded mouse testis using Nanog (GB11331) at dilution of 1:500




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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