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GB11528 Anti -Alkaline Phosphatase Rabbit pAb Antibody

Immunogen: Recombinant protein corresponding to Mouse Alkaline Phosphatase
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11528
  • Servicebio

Description

Product Information


Description

Alkaline Phosphatase rabbit polyclonal

Protein full name

Alkaline phosphatase, tissue-nonspecific isozyme

Synonyms

ALPL, AP-TNAP, APTNAP, HOPS, TNAP,

TNSALP, Alkaline phosphatase,

liver/bone/kidney, TNALP

Immunogen

Recombinant protein corresponding to

Mouse Alkaline Phosphatase

Isotype

IgG

Purity

Affinity purification

Predicted MW.

57 kDa

Observed MW.

55 kDa

Uniprot ID

P05186, P09242, P08289




Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, kidney , cartilage




Background


Intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific) all belong to alkaline phosphatases. the tissue-nonspecific form is located on chromosome 1. The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. This enzyme has been linked directly to hypophosphatasia disorder characterized by hypercalcemia and skeletal defects. Alkaline phosphatase allows for mineralization of calcium and phosphorus by bones and teeth. ALPL gene mutation leads to insufficient enzyme tissue-nonspecific alkaline phosphatase and allows for an accumulation of chemicals such as inorganic pyrophosphate to indirectly cause elevated calcium levels in the body and lack of bone calcification.



Images



image.png

Western blot analysis of Alkaline Phosphatase (GB11528) at dilution of 1: 1000.

Lane 1: Mouse liver tissue lysate

Lane 2: Mouse kidney tissue lysate

Lane 3: Mouse cartilage tissue lysate

Lane 4: Rat liver tissue lysate

Lane 5: Rat kidney tissue lysate

Lane 6: Rat cartilage tissue lysate

image.png

Western blot analysis of Alkaline Phosphatase (GB11528) at dilution of 1:1000.

Lane 1: Human colon tissue lysate

Lane 2: Human liver tissue lysate

Lane 3: Mouse liver tissue lysate

Lane 4: Mouse kidney tissue lysate

Lane 5: Mouse bone tissue lysate

Lane 6: Rat liver tissue lysate

Lane 7: Rat kidney tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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