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GB11531 Anti -SQSTM1 P62 NADGP Sequestosome 1 FTDALS3 Rabbit Polyclonal Antibody for WB

Immunogen: KLH conjugated Synthetic peptide corresponding to Human SQSTM1
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11531
  • Servicebio

Description

Product Information


Description

SQSTM1 / p62 rabbit polyclonal

Protein full name

Sequestosome-1

Synonyms

SQSTM1, A170, OSIL, PDB3, ZIP3, P60,

P62, P62B, FTDALS3, Sequestosome 1,

NADGP, DMRV

Immunogen

KLH conjugated Synthetic peptide

corresponding to Human SQSTM1

Isotype

IgG

Purity

Affinity purification

Predicted MW.

48 kDa

Observed MW.

62 kDa

Uniprot ID

Q13501, Q64337, O08623




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

HeLa, HIN3T3, HepG2, MCF7




Background


The product of SQSTM1 gene, Sequestosome-1, also kown as ubiquitin-binding protein p62, is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. By interacting with GATA4 and targeting it for degradation, it can inhibit GATA-4 associated senescence and senescence-associated secretory phenotype.

   



Images



image.png

Western blot analysis of SQSTM1 / p62 (GB11531) at dilution of 1: 1000 (upper) or β-actin (lower).

Lane 1: HeLa (untreated) cell lysate

Lane 2: HeLa (treated with Chloroquine, 50 μM, overnight ) cell lysate

Lane 3: NIH3T3 (untreated) cell lysate

Lane 4: NIH3T3 (treated with Chloroquine, 50 μM, overnight ) cell lysate

Lane 5: HepG2 cell lysate

Lane 6: MCF7 cell lysate

Lane 7: A549 cell lysate

Lane 8: L929 cell lysate

Lane 9: PC12 cell lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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