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GB11532 IHC IF Anti -Cleaved- Caspase-3 CASP3 CPP32 SCA-1 Rabbit Polyclonal Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse caspase3
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11532
  • Servicebio

Description

Product Information


Description

Cleaved- Caspase-3 rabbit polyclonal

Protein full name

Caspase-3

Synonyms

CASP3, CPP32, CPP32B, SCA-1, Caspase 3, Caspase-3 subunit p12, PARP cleavage protease

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse caspase3

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Uniprot ID

P70677, P42574, P55213




Applications


Applications

Species

Dilution

Positive Tissue

IHC

Human, Mouse, Rat

1: 500-1: 1000

mouse tumor, A549 mice tumor, MCF7 mice tumor

IF

Human

1: 500-1: 1000

esophagus, stomach, colon




Background


Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.




Images



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Immunohistochemistry analysis of paraffin-embedded A549 in mice tumor using Cleaved- Caspase-3(GB11532) at dilution of

1: 1000, untread (left), treated with the control peptide antigen (right) .

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Immunohistochemistry analysis of paraffin-embedded MCF-7 in mice tumor using Cleaved- Caspase-3 (GB11532) at dilution of

1: 1000, untread (left), treated with the control peptide antigen (right) .

blob.png blob.png

Immunohistochemistry analysis of paraffin-embedded mouse tumor using Cleaved- Caspase-3 (GB11532) at dilution of

1: 1000, untread (left), treated with the control peptide antigen (right) .


Immunofluorescent analysis of paraformaldehyde-fixed human esophagus using Cleaved-Caspase3 (GB11532) at dilution of 1:500 .

Immunofluorescent analysis of paraformaldehyde-fixed human stomach using Cleaved-Caspase3 (GB11532) at dilution of 1:1000 .

Immunofluorescent analysis of paraformaldehyde-fixed human colon using Cleaved-Caspase3 (GB11532) at dilution of 1:500 .




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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