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GB11536 Anti -Bag3 BIS CAIR-1 MFM6 Rabbit Polyclonal Antibody for WB

Immunogen: Recombinant protein corresponding to human Bag3
Isotype: IgG
Purity: Affinity purification

 

ul:
  • GB11536
  • Servicebio

Description

Product Information


Description

Bag3 rabbit polyclonal

Protein full name

BAG family molecular chaperone regulator 3

Synonyms

BAG3, BAG-3, BIS, CAIR-1, MFM6, BCL2

associated athanogene 3, Docking protein CAIR-1

Immunogen

Recombinant protein corresponding to

human Bag3

Isotype

IgG

Purity

Affinity purification

Predicted MW.

62 KDa

Observed MW.

86 KDa

Uniprot ID

O95817, Q9JLV1




Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

heart, muscle,

brain




Background


BAG proteins compete with Hip-1 for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.




Images



1533019053129925.png

Western blot analysis of Bag3 (GB11536) at dilution of 1:800.

Lane 1:HepG2 cell lysate

Lane 2:MCF7 cell lysate

Lane 3:A549 cell lysate

Lane 4:Mouse heart tissue lysate

Lane 5:Mouse muscle tissue lysate

Lane 6:Mouse brain tissue lysate

Lane 7:Rat heart tissue lysate

Lane 8:Rat muscle tissue lysate

Lane 9:Rat brain tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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