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Anti -Sarcomeric Alpha Actinin Rabbit pAb

  • Servicebio

Description

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Product Information

Description

Sarcomeric Alpha Actinin rabbit polyclonal

Protein full name

Alpha-actinin-2

Synonyms

ACTN2, CMD1AA, CMH23, Actinin alpha 2, alpha Actinin 2

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse alpha Actinin 2

Isotype

IgG

Purity

Affinity purification

Predicted MW.

104 kDa

Observed MW.

104 kDa

Subcellular location

Cytoplasm

Uniprot ID

Q9JI91



Applications

Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 2000

heart, muscle, lung

IHC

Mouse, Rat

1: 500-1: 1000

heart, muscle

IF

Mouse, Rat

1: 500-1: 1000

heart, muscle

IEM

Mouse, Rat

1: 50

heart



Background

The primary function of alpha-actinin 2 is to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. It is clear from studies by Hampton etal. that this crosslinking can assume a variety of conformations, with preferences for 60° and 120° angles. Alpha-actinin 2 also functions in docking signaling molecules at Z-discs, and additional studies have also implicated alpha-actinin 2 in the binding of cardiac ion channels, Kv1.5 in particular.


Images

image.png

Western blot analysis of alpha Actinin 2 (GB11555) at dilution of 1: 2000.

Lane 1: Mouse heart tissue lysate

Lane 2: Mouse muscle tissue lysate

Lane 3: Mouse lung tissue lysate

Lane 4: Rat heart tissue lysate

Lane 5: Rat muscle tissue lysate


image.png

Immunohistochemistry analysis of paraffin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 500

image.png

Immunohistochemistry analysis of paraffin-embedded mouse muscle using alpha Actinin 2 (GB11555) at dilution of 1: 1000

image.png

Immunohistochemistry analysis of paraffin-embedded rat muscle using alpha Actinin 2 (GB11555) at dilution of 1: 500.

image.png

Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 50.

A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.

Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 50.

A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.

Immunohistochemistry analysis of paraffin-embedded rat heart using alpha Actinin 2 (GB11555) at dilution of 1: 1000

Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 50.

A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50.


Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


NOTE: This product is intended for research only.

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