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GB11562 Anti -GAD65 GAD2 GAD2 DCE 2 Rabbit pAb Antibody

Immunogen: Recombinant protein corresponding to Mouse GAD65
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11562
  • Servicebio

Description

Product Information


Description

GAD65 rabbit polyclonal

Protein full name

Glutamate decarboxylase 2

Synonyms

GAD2, GAD65, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, DCE 2, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65

Immunogen

Recombinant protein corresponding to Mouse GAD65

Isotype

IgG

Purity

Affinity purification

Predicted MW.

65 kDa

Observed MW.

65 kDa

Uniprot ID

Q05329, Q05683, P48320




Applications


applications

species

dilution

positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, cerebellum, hippocampus




Background


Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD67 and GAD65). GAD67 and GAD65 are expressed in the brain where GABA is used as a neurotransmitter, and they are also expressed in the insulin-producing β-cells of the pancreas. GAD65, synthesizes GABA for neurotransmission, and therefore is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter VGAT, which, as a complex, helps package GABA into vesicles for release during neurotransmission.




Images



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Western blot analysis of GAD65

(GB11562) at dilution of 1: 1000.

Lane 1: Hela cell lysate

Lane 2: U87 cell lysate

Lane 3: Jurkat cell lysate

Lane 4: Mouse brain tissue lysate

Lane 5: Mouse cerebellum tissue lysate

Lane 6: Mouse hippocampus tissue lysate

Lane 7: Rat brain tissue lysate

Lane 8: Rat cerebellum tissue lysate

Lane 9: Rat hippocampus tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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