PHONE

+86(027) 5111 3188

Share to:

GB11566 Anti -SHC P46 p66 p52SHC Rabbit Polyclonal Antibody

Immunogen: Recombinant protein corresponding to Mouse SHC
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11566
  • Servicebio

Description

Product Information


Description

SHC rabbit polyclonal

Protein full name

SHC-transforming protein 1

Synonyms

SHC1, SHC, SHCA, SHC adaptor protein 1, p46, p52, p52 SHC, p52SHC, p66, p66 SHC

Immunogen

Recombinant protein corresponding to

Mouse SHC

Isotype

IgG

Purity

Affinity purification

Predicted MW.

63 kDa

Observed MW.

63 kDa

Uniprot ID

P29353, Q5M824, P98083




Applications


applications

species

dilution

positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, heart, brain




Background


SHC-transforming protein 1 is a protein that in humans is encoded by the SHC1 gene. SHC has been found to be important in the regulation of apoptosis and drug resistance in mammalian cells. The gene SHC1 is located on chromosome 1 and encodes 3 main protein isoforms: p66SHC, p52SHC and p46SHC. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis.




Images



1534950450376398.png

Western blot analysis of SHC

(GB11566) at dilution of 1: 1000

Lane 1: Hela cell lysate

Lane 2: U251 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: Mouse liver tissue lysate

Lane 5: Mouse heart tissue lysate

Lane 6: Mouse brain tissue lysate

Lane 7: Rat liver tissue lysate

Lane 8: Rat heart tissue lysate

Lane 9: Rat brain tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.