PHONE

+86(027) 5111 3188

Share to:

GB11574 Anti -Chk2 Rabbit pAb Antibody

Immunogen: Recombinant protein corresponding to Mouse Chk2
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11574
  • Servicebio

Description

Product Information


Description

Chk2 rabbit polyclonal

Protein full name

Serine/ threonine-protein kinase Chk2

Synonyms

CHEK2, CDS1, Chk2, HuCds1, LFS2, PP1425, RAD53, hCds1, Checkpoint kinase 2

Immunogen

Recombinant protein corresponding to

Mouse Chk2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

61 kDa

Observed MW.

61 kDa

Uniprot ID

O96017, Q9Z265




Applications


applications

species

dilution

positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

kidney, colon, thymus

IHC

Human, Mouse, Rat

1: 500-1: 1000

colon, intestine

IF

Human, Mouse, Rat

1: 500-1: 1000

colon, intestine




Background


CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2. CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2. Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs).




Images



image.png

Western blot analysis of Chk2

(GB11574) at dilution of 1: 800

Lane 1: HCT116 cell lysate

Lane 2: Mouse kidney tissue lysate

Lane 3: Mouse colon tissue lysate

Lane 4: Mouse thymus tissue lysate

Lane 5: Rat kidney tissue lysate

Lane 6: Rat colon tissue lysate

Lane 7: Rat thymus tissue lysate


image.png

Immunohistochemistry analysis of paraffin-embedded human colon using

Chk2 (GB11575) at dilution of 1: 1000


image.png

Immunohistochemistry analysis of paraffin-embedded mouse intestine using

Chk2 (GB11575) at dilution of 1: 1000





Storage



Storage

Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles

Storage Buffer

PBS with 0.02%sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.