PHONE

+86(027) 5111 3188

Share to:

Anti -Myosin Phosphatase Rabbit PAb for Western Blot

Specification:100uL
  • GB11672
  • Servicebio
  • Servicebio

Description

Product Information


Description

Myosin Phosphatase rabbit polyclonal

Protein full name

Protein phosphatase 1 regulatory subunit 12A

Synonyms

Ppp1r12a, PPP1R12A, M130, MBS, MYPT1, protein phosphatase 1 regulatory subunit 12A, Myosin Phosphatase

Immunogen

Recombinant protein corresponding to Mouse Myosin Phosphatase

Isotype

IgG

Purity

Affinity purification

Predicted MW.

115 kDa

Observed MW.

130 kDa

Uniprot ID

O14974, Q9DBR7, Q10728




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

lung, brain




Background


Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.



Images



内页.jpg

Western blot analysis of Myosin Phosphatase (GB11672) at dilution of 1: 1000

Lane 1: HeLa cell lysate

Lane 2: MCF7 cell lysate

Lane 3: A549 cell lysate

Lane 4: Mouse lung tissue lysate

Lane 5: Mouse brain tissue lysate

Lane 6: Rat lung tissue lysate

Lane 7: Rat brain tissue lysate





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

downLoadImgLaboratory(1)Slice 13Slice 10Slice 12

Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.