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Anti -FIH1 Rabbit pAb

  • Servicebio

Description

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Product Information

Description

FIH1 rabbit polyclonal

Protein full name

Hypoxia-inducible factor 1-alpha inhibitor

Synonyms

FIH 1, FIH-1 , FIH1, HIF1AN, Hypoxia inducible factor 1 alpha inhibitor, Hypoxia inducible factor 1 alpha subunit inhibitor

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse FIH1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

40 kDa

Observed MW.

43 kDa

Uniprot ID

Q8BLR9, Q9NWT6



Applications

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, heart, muscle

IHC

Human

1: 3000-1: 6000

colon, colon cancer

IF

Human

1: 1500-1: 3000

colon, colon cancer



Background

HHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.


Images

image.png

Western blot analysis of FIH1

(GB11843) at dilution of 1: 1000

Lane 1: HCT116 cell lysate

Lane 2: PC12 cell lysate

Lane 3: A20 cell lysate

Lane 4: Mouse brain tissue lysate

Lane 5: Mouse heart tissue lysate

Lane 6: Mouse muscle tissue lysate

Lane 7: Rat brain tissue lysate

Lane 8: Rat heart tissue lysate

Lane 9: Rat muscle tissue lysate


image.png

Immunohistochemistry analysis of paraffin-embedded human colon using

FIH1 (GB11843) at dilution of 1: 3000


image.png

Immunohistochemistry analysis of paraffin-embedded human colon cancer

using FIH1 (GB11843) at dilution of 1: 3000



Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


NOTE: This product is intended for research only.

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