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GB11888 Anti -XIAP Rabbit pAb Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse XIAP
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11888
  • Servicebio

Description

Product Information


Description

XIAP rabbit polyclonal

Protein full name

E3 ubiquitin-protein ligase XIAP

Synonyms

Xiap, XIAP, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3, X-linked inhibitor of apoptosis

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse XIAP

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Uniprot ID

P98170




Applications


Applications

Species

Dilution

Positive Tissue

IHC

Human

1: 500-1: 1000

lung cancer,

colon cancer

IF

Human

1: 250-1: 500

lung cancer,

colon cancer




Background


X-linked inhibitor of apoptosis protein (XIAP) is a protein that stops apoptotic cell death. XIAP is a member of the inhibitor of apoptosis family of proteins (IAP). XIAP stops apoptotic cell death that is induced either by viral infection or by overproduction of caspases. Caspases are the enzymes primarily responsible for cell death. XIAP binds to and inhibits caspase 3, 7 and 9. The BIR2 domain of XIAP inhibits caspase 3 and 7, while BIR3 binds to and inhibits caspase 9. The RING domain utilizes E3 ubiquitin ligase activity and enables IAPs to catalyze ubiquination of self, caspase-3, or caspase-7 by degradation via proteasome activity. However, mutations affecting the RING Finger do not significantly affect apoptosis, indicating that the BIR domain is sufficient for the protein’s function. When inhibiting caspase-3 and caspase-7 activity, the BIR2 domain of XIAP binds to the active-site substrate groove, blocking access of the normal protein substrate that would result in apoptosis.



Images


Immunohistochemistry analysis of paraffin-embedded human lung cancer

using XIAP (GB11888) at dilution of 1: 800


Immunohistochemistry analysis of paraffin-embedded human colon cancer

using XIAP (GB11888) at dilution of 1: 800





Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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