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GB11889 Anti -ATG4A Rabbit pAb Antibody

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse ATG4A
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11889
  • Servicebio

Description

Product Information


Description

ATG4A rabbit polyclonal

Protein full name

Cysteine protease ATG4A

Synonyms

Atg4a, ATG4A, APG4A, AUTL2, Autophagy related 4A cysteine peptidase

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse ATG4A

Isotype

IgG

Purity

Affinity purification

Predicted MW.

45 kDa

Observed MW.

48 kDa

Uniprot ID

Q8WYN0, Q8C9S8




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

skeletal muscle, pancreas, liver




Background


Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.




Images



image.png

Western blot analysis of ATG4A

(GB11889) at dilution of 1: 1000

Lane 1: K562 cell lysate

Lane 2: U251 cell lysate

Lane 3: LO2 cell lysate

Lane 4: Mouse skeletal muscle tissue lysate

Lane 5: Mouse pancreas tissue lysate

Lane 6: Mouse liver tissue lysate

Lane 7: Rat skeletal muscle tissue lysate

Lane 8: Rat pancreas tissue lysate





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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