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GB11896 Anti -Smac / Diablo Rabbit pAb Antibody for WB

Immunogen: Recombinant protein corresponding to Mouse Smac
Isotype: IgG
Purity: Affinity purification
ul:
  • GB11896
  • Servicebio

Description

Product Information



Description

Smac rabbit polyclonal

Protein full name

Diablo homolog, mitochondrial

Synonyms

Diablo, DIABLO, DFNA64, SMAC, Diablo, Diablo homolog,diablo IAP-binding mitochondrial protein, Smac

Immunogen

Recombinant protein corresponding to Mouse Smac

Isotype

IgG

Purity

Affinity purification

Predicted MW.

27 kDa

Observed MW.

21 kDa

Uniprot ID

Q9NR28, Q9JIQ3




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

heart, brain, kidney




Background


Diablo homolog (DIABLO) is a mitochondrial protein encoded by the DIABLO gene. SMAC is a mitochondrial protein that promotes cytochrome c- and TNF receptor-dependent activation of apoptosis by inhibiting the effect of IAP–a protein that negatively regulates apoptosis, or programmed cell death. SMAC is normally a mitochondrial protein localized to the mitochondrial intermembrane space, but it enters the cytosol when cells undergo apoptosis. Through the intrinsic pathway of apoptosis, BCL-2 proteins like BAK and BAX form a pore in the outer mitochondrial membrane, leading to mitochondrial membrane permeabilization and the release of both cytochrome c and SMAC. While cytochrome c directly activates APAF1 and caspase 9, SMAC binds IAPs, such as XIAP and cIAP proteins, to inhibit their caspase-binding activity and allow for caspase activation of apoptosis. SMAC is ubiquitously expressed in many cell types, implicating it in various biological processes involving apoptosis. Currently, nonapoptotic functions for SMAC remain unclear.




Images



image.png

Western blot analysis of Smac

(GB11896) at dilution of 1: 1000

Lane 1: MCF7 cell lysate

Lane 2: HepG2 cell lysate

Lane 3: Mouse heart tissue lysate

Lane 4: Mouse liver tissue lysate

Lane 5: Mouse kidney tissue lysate

Lane 6: Rat heart tissue lysate

Lane 7: Rat liver tissue lysate

Lane 8: Rat kidney tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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