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Anti -Ube2N / Ubc13 Rabbit pAb for WB IHC IF

Specification: 100uL
  • GB11986
  • Servicebio
  • Servicebio

Description

Product Information


Description

Ube2N rabbit polyclonal

Protein full name

Ubiquitin-conjugating enzyme E2 N

Synonyms

UbCH ben, Ube2N, ubiquitin carrier protein N, Ubiquitin protein ligase N, BLU, Bendless-like ubiquitin-conjugating enzyme, MGC131857, MGC8489

Immunogen

Recombinant protein corresponding to Mouse Ube2N

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Predicted MW.

17 kDa

Observed MW.

17 kDa

Uniprot ID

P61088, P61089, Q9EQX9




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

testis, small intestine, spinal cord

IHC

Human, Mouse, Rat

1: 500-1: 1000

colon cancer, ovarian cancer, spinal cord, brain

IF

Human, Mouse, Rat

1: 250-1: 500

colon cancer, ovarian cancer, spinal cord, brain




Background


UBC13 is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. Existing as a heterodimer with UBE2V2, UBC13 catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. Additionally, UBC13 mediates the transcription of several target genes and is thought to play a role in cell cycle progression, cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.




Images


image.png

Western blot analysis of Ube2N (GB11986) at dilution of 1: 1000

Lane 1: 293T cell lysate

Lane 2: SH-SY5Y cell lysate

Lane 3:Mouse testis tissue lysate

Lane 4: Mouse small intestine tissue lysate

Lane 5: Mouse spinal cord tissue lysate

Lane 6: Rat testis tissue lysate

Lane 7: Rat small intestine tissue lysate

Lane 8: Rat spinal cord tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded human colon cancer using Ube2N (GB11986) at dilution of 1: 600

image.png

Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using Ube2N (GB11986) at dilution of 1: 600

image.png

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Ube2N (GB11986) at dilution of 1: 600

image.png

Immunohistochemistry analysis of paraffin-embedded rat brain using Ube2N (GB11986) at dilution of 1: 600




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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