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Anti -PDCD4 Rabbit pAb for WB IHC IF

Specification: 100uL
  • GB11991
  • Servicebio
  • Servicebio

Description

Product Information


Description

PDCD4 rabbit polyclonal

Protein full name

Programmed cell death protein 4

Synonyms

Topoisomerase-inhibitor suppressed protein, Death up-regulated gene protein, MGC33046, MGC33047, Protein MA-3, Ma3, Tis, Dug, H731, Neoplastic transformation inhibitor, Nuclear antigen H731, Protein 197/15a, RP11 348N5.4, Nuclear antigen H731-like

Immunogen

Recombinant protein corresponding to

Mouse PDCD4

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

52 kDa

Observed MW.

64 kDa

Uniprot ID

Q53EL6, Q61823, Q9JID1




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, thymus, colon

IHC

Human, Rat

1: 800-1: 1600

colon cancer, spleen, breast, thymus

IF

Human, Rat

1: 400-1: 800

colon cancer, spleen, breast, thymus




Background


The Pdcd4 gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells.Two transcripts encoding different isoforms have been identified. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.




Images


image.png

Western blot analysis of PDCD4 (GB11991) at dilution of 1: 1000

Lane 1: T47-D cell lysate

Lane 2: Mouse Spleen tissue lysate

Lane 3: Mouse thymus tissue lysate

Lane 4: Mouse colon tissue lysate

Lane 5: Rat thymus tissue lysate

Lane 6: Rat colon tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded human colon cancer using PDCD4 (GB11991) at dilution of 1: 800

image.png

Immunohistochemistry analysis of paraffin-embedded human breast using PDCD4 (GB11991) at dilution of 1: 800

image.png

Immunohistochemistry analysis of paraffin-embedded rat spleen using PDCD4 (GB11991) at dilution of 1: 800

image.png

Immunohistochemistry analysis of paraffin-embedded rat thymus using PDCD4 (GB11991) at dilution of 1: 800




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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