PHONE

+86(027) 5111 3188

Share to:

Anti -Brd4 Rabbit PAb for Western Blot

Specification: 100uL
  • GB11999
  • Servicebio
  • Servicebio

Description

Product Information


Description

Brd4 rabbit polyclonal

Protein full name

Bromodomain-containing protein 4

Synonyms

Mitotic chromosome-associated protein, HUNK1bromodomain-containing protein 4, HUNK1,MCAP, Brd4, Mcap

Immunogen

Recombinant protein corresponding to Mouse Brd4

Isotype

IgG

Purity

Affinity purification

Predicted MW.

156 kDa

Observed MW.

180 kDa

Uniprot ID

O60885, Q9ESU6, D3ZGX8




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, lymph node




Background


BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms, longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.




Images


image.png

Western blot analysis of Brd4 (GB11999) at dilution of 1: 1000

Lane 1: Hela cell lysate

Lane 2: Hct116 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: Mouse liver tissue lysate

Lane 5: Mouse lymph node tissue lysate

Lane 6: Rat liver tissue lysate




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

downLoadImgSlice 10Laboratory(1)Slice 13Slice 12

Contact us

Quick Links

Product Category

Get In Touch

 5th Floor, 22 Building, Biopark, Gaoxin 2nd Road No. 388, East Lake High-Tech Developing Zone, Wuhan, Hubei, China 430079
 +86(027) 5111 3188
Copyright © 2021  Wuhan Servicebio Technology Co., Ltd.