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Anti -GAPDH Mouse MAb Loading Control Primary Antibody

specification:100ul
  • GB12002
  • Servicebio
  • Servicebio

Description

Product Information



Description

GAPDH mouse monoclonal

Protein full name

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms

GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse GAPDH

Isotype IgG
Purity Affinity purification
Predicted MW. 37 kDa
Observed MW. 37 kDa
Subcellular location

Cytoplasm,Nucleus,Membrane

Uniprot ID

P04406,P04797,P16858




Applications



Applications

WB

IHC/IF
Species

Human,Rat,Mouse

Human,Rat,Mouse

Dilution 1:1000

1:200

Positive tissue Hela,HepG2,jurkat,lung,kidney,brain Liver cancer,brain




Background



Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.




Images



image.png

Western blot analysis of GAPDH (GB12002) at dilution of 1:1000.

Lane 1:Hela cell lysate

Lane 2:HepG2 cell lysate

Lane 3:Jurkat cell lysate

Lane 4:Mouse lung tissue lysate

Lane 5:Mouse kidney tissue lysate

Lane 6:Mouse brain tissue lysate

Lane7: Rat lung tissue lysate

Lane 8:Rat kidney tissue lysate

Lane 9:Rat brain tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAPDH (GB120002) at dilution of 1:200

image.png

Immunohistochemistry analysis of paraffin-embedded mouse brain using GAPDH (GB120002) at dilution of 1:200




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE: This product is intended for research only.

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